spectrophotometer

spectrophotometer
The spectrophotometer measures the amount of light transmitted through the sample
By using an equation (Beers law), it converts the transmittance data to an absorbance value

• Absorbance, Transmittance, and Reflection. Aspectrophotometer measures how light interacts with atoms or molecules in a sample.
Quantitative Spectroscopy
Beer’s Law
Al1 = el1bc
e is molar absorptivity (unique for a given compound at l1)
b is path length
c concentration
Lamber s law
A ? b
A ? C
A=a b c
a=A / b c
A1/B 1C1=A 2 /B2 C2
A 1 /C 1 =A2/C2
CU =AU/AS *CS
CU =AU * CS /AS =AU *F
Absorption Measurement Colorimeter with
1-filter Spectrophotometer
2-Prism
3-Diffraction grating


• Parts of a Spectrophotometer
• Lamp
• Prism
• Sample holder
• Display
• Beer’s Law and Spectrophotometry


• spectrophotometer
• The spectrophotometer measures the amount of light transmitted through the sample
• By using an equation (Beers law), it converts the transmittance data to an absorbance value
•
• Absorbance, Transmittance, and Reflection. Aspectrophotometer measures how light interacts with atoms or molecules in a sample.
• Quantitative Spectroscopy
• Beer’s Law
• Al1 = el1bc
• e is molar absorptivity (unique for a given compound at l1)
• b is path length
• c concentration
•








Spectral Distribut