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penicillium

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الكلية كلية الطب     القسم  الاحياء المجهرية     المرحلة 3
أستاذ المادة عبير ظاهر ناجي حمزة الحسناوي       4/28/2011 9:26:05 AM

practical mycology  lab-3

penicillium (show the students the slide of penicillium)

penicillium are comparable to aspergillus. the genus penicillium falls into the order eurotiales. in this order, organisms produce asci within cleistothecia. penicillium is often reffered to as deuteromycetes, or fungi imperfecti. the name penicillium comes from the word "brush" this refers to the appearance of spores in penicillium.

one species, penicillium chrysogenum , is classified as a psychrotrophic microorganism. this species was one of the best lipase producers among other fungi they studied in the arctic tundra. penicillium chrysogenum has high enzymatic activity and has the ability to produce alpha-amylase.    

with the exception of penicillium marneffei, which is thermally dimorphic, penicillium are filamentous fungi. they have branched conidiospores. conidia are round and unicellular. glucans are common in the cell walls of penicillium species. penicillium species tend to have small hyphae. this makes protoplasmic movement difficult to detect. the small hyphae also lead to smaller peripheral growth zones. penicillium spores have a hydropinginginghobic surface. however, they are capable of being wetted this is necessary for germination to occur. penicillium are osmotolerant, meaning that although they grow better with high water levels, they are able to tolerate low water potential.

penicillium species are heterotrophic. the pathogenic species feed off of the fruit they destroy.

penicillium produces asexually, and are unable to sporulate when submerged. however, they begin reproduction easily when hyphae emerge into a gas phase. no species has the exact same method of reproduction. each is classified based on the way it reproduces. for example, in some species, conidia are borne on phialidies, which group out of the conidiophore. in others the conidiophore bears metullae, where phialidies are borne. still in others the conidiophore may branch out before bearing metullae. branching may or may not be symmetrical, depending on species. sporulation is not stimulated by changes in oxygen, carbon dioxide, or water loss. instead, it is associated with change in physical environment at the hyphal surface. there is no specific method for ascospore dispersal.

 

 

pencillium fungi are versatile and opportunistic. they are post-harvest pathogens. penicillium species are one of the most common causes of fungal spoilage in fruits and vegetables. penicillium italicum and penicillium digitatum are the most common attackers of citrus fruits, while penicillium expansum is known to attack apples. p. digitatum works by producing ethylene to accelerate ripening. it the covers the fruit with green conidia, causing the fruit to shrivel and dry out. p. italicum causes slimy rot and produces blue-green conidia. these species like cooler temperatures, which explains why they are usually found on foods left too long in the refrigerator. many species produce mycotoxins for example, p. expansum produces one called patulin. most of these species resemble each other in color characteristics, style of decay, and infection symptoms they fall under a general category called blue mold. p. expansum is one of the most aggressive species. these fungi live a long time and are quite durable, even under adverse conditions. sometimes, p. italicum and p. expansum will adhere to each other to create synnemata. synnemata also occurs in penicillum claviforme. penicillium growth typically occurs as a result of wound infections in produce. the most common treatment is to use fungicide on harvested produce. penicillium species attack more than just fruit. for example, penicillium verrucosum grows on cereal products.

one species, penicillium marneffei, is rare to most of the world. it is endemic to southeast asia and infects bamboo rats. it can be spread to humans, especially immunosuppressed individuals.

however, penicillium is not merely a harmful fungus. it also has many useful species. for example, penicillium roquefortii is used to make blue cheese. the color of the cheese comes from the spores (conidia) of the fungus. the spores are injected into the cheese curd during fermentation. penicillium camambertii is another species used to produce cheese.

some penicillium species actually help prevent fungal decay as opposed to producing it. penicillium chrysogenum produces glucose oxidase, which is used as a preservative in fruit juices.

one of the things penicillium is most famous for is the drug penicillin. it was used to create the first antibiotic. the originial strain, penicillium notatum, was discovered in 1920 by sir alexander fleming. however, it was replaced with penicillium chrysogenum, a more productive species, which is now the species used in manufacturing penicillin.

 

 


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