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  Occurrence and detection of extended-spectrum B- lactamases in Klebsiella isolates in Hilla, Iraq Alaa H. Al-Charrakh, Samira Y. Yousif and Hussein S. Al-Janabi

  Abstract 

 Extended-spectrum beta-lactamases (ESBLs) have been found in many pathogenic gram-negative bacteria, butthey are most common in nosocomial isolates of Klebsiella pneumoniae, giving a proportion of 75% of ESBL-producing strains. A total of 88 Klebsiella pneumoniae isolates were recovered from different environmental and clinical samples in Hilla/Iraq during the period from January to July 2003. Primary screening of B-Lactam resistant isolates showed that 73.8% (65 strains) were resistant to -Lactam antibiotics. 58.4% of these strains were B- lactamase-producers. All the B- lactamase-producing Klebsiella strains showed multiple-drug resistance to least 8 antibiotics. Klebsiella strains were also tested for their ability to produce extended-spectrum beta-lactamases (ESBLs) using three methods.

 Determination of minimum inhibitoryconcentration (MIC) with and without clavulanate was the most accurate method for detection of ESBLproducingisolates, by which 8 (21%) ESBL-producing isolates were detected. Plasmid profile of Klebsiella strains (including ESBL-producers) was detected.   The genes encoding for the production of ESBLs and resistance to penicillin, ampicillin, amoxicillin, tetracycline, refampin, and erythromycin were located on conjugative plasmids whereas genes encoding for resistance to cephalothin, cefazolin, cephalexin and gentamycin were located on the chromosome.To our knowledge, the present study is the first to investigate the occurrence of ESBLs and detection of genetic factors controlling ESBL production in Klebsiella isolates in the area of the study.


  • وصف الــ Tags لهذا الموضوع
  • Antimicrobial resistance, -lactamases, ESBLs, plasmid profile, Klebsiella pneumoniae.

هذه الفقرة تنقلك الى صفحات ذات علاقة بالمقالات الاكاديمية ومنها الاوراق البحثية المقدمة من قبل اساتذة جامعة بابل وكذلك مجموعة المجلات العلمية والانسانية في الجامعة وعدد من المدنات المرفوعة من قبل مشرف موقع الكلية وهي كالاتي:

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